Genetic screen on cell line
CRISPR’it core facility provides a full support to help you design and perform your custom CRISPR-based genetic screen on cell line:
- Positive and Negative screen
- In positive-selection screens, the aim is to identify genes whose mutation, silencing or overexpression, gives a positive advantage over a given selective pressure (e.g., a drug). Positive screens are typically used to study the mechanisms of drug resistance in cancer cells. They can also serve in the study of various cellular processes as long as a positive pressure is robust (e.g., antibiotic selection, fluorescence-activated cell sorting - FACS).
- In contrast, negative-selection screens aim at identifying genes that are essential for survival or proliferation under defined conditions. Such screens are often used to identify context-dependent gene essentiality (e.g., synthetic lethal dependencies).
- Genome Wide or custom Library
- The facility already work with several GW commercial library available on Addgene (Sabatini, Brunello, Weissman, Calabrese, etc)
- We also offer to clone your custom library with restricted number of genes when prior gene selection is possible
- Knock-Out screen (CRISPR-KO), inhibition screen (CRISPRi) or activation screen (CRISPRa)
- Regular SpCas9 to perform gene inactivation screen
- dCas9-KRAB screen for transcriptional inactivation of genes
- dCas9-VPR screen for transcriptional activation of genes
In parallel of “classical” screens previously mentioned, CRISPR’it core facility is trying to improve their services with R&D activities (see following part) and can adapt to specific demands you may have.
- CRISPR’it core facility is part of the network Core For Life that regroup core facilities around Europe.