Genetic screen on cell line

CRISPR’it core facility provides a full support to help you design and perform your custom CRISPR-based genetic screen on cell line:


  • Positive and Negative screen
    • In positive-selection screens, the aim is to identify genes whose mutation, silencing or overexpression, gives a positive advantage over a given selective pressure (e.g., a drug). Positive screens are typically used to study the mechanisms of drug resistance in cancer cells. They can also serve in the study of various cellular processes as long as a positive pressure is robust (e.g., antibiotic selection, fluorescence-activated cell sorting - FACS).
    • In contrast, negative-selection screens aim at identifying genes that are essential for survival or proliferation under defined conditions. Such screens are often used to identify context-dependent gene essentiality (e.g., synthetic lethal dependencies).


  • Genome Wide or custom Library
    • The facility already work with several GW commercial library available on Addgene (Sabatini, Brunello, Weissman, Calabrese, etc)
    • We also offer to clone your custom library with restricted number of genes when prior gene selection is possible


  • Knock-Out screen (CRISPR-KO), inhibition screen (CRISPRi) or activation screen (CRISPRa)
    • Regular SpCas9 to perform gene inactivation screen
    • dCas9-KRAB screen for transcriptional inactivation of genes
    • dCas9-VPR screen for transcriptional activation of genes


In parallel of “classical” screens previously mentioned, CRISPR’it core facility is trying to improve their services with R&D activities (see following part) and can adapt to specific demands you may have.


  • CRISPR’it core facility is part of the network Core For Life that regroup core facilities around Europe.


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