The project will be developed under the supervision of Dr. Olivier Lantz, director of the team "Innate and CD4+ T cells in cancer" (https://science.institut-curie.org/research/integrated-biology/u932-immunity-and-cancer/team-lantz/). The aim of the team is to characterize the biology of T cells, their interaction with other cells of the immune system, and their role in cancer, in both humans and through mice models. The open position is in line with recent developments at the interface between Immunology and Cancer (Bigot et al. Cancer Discovery 2021).
The techniques used routinely are: analysis and cell sorting by multiparametric cytometry, (co-)culture of primary cells and cell lines, cellular immunology, Elispots, protein assays (Elisa, CBA, luminex...), ... etc. Many studies are performed using samples (blood, plasma, tissues) from patients, and/or using experimental models in mice.
The laboratory is located on the Institut Curie campus in the center of Paris. The building is equipped with state-of-the-art facilities (imaging, proteomics, animal facility, genomics and cytometry platforms). The Institute hosts about 1500 scientists, including many foreign postdoctoral fellows.
Bigot J, Lalanne AI, Lucibello F,…Lantz O. Splicing Patterns in SF3B1-Mutated Uveal Melanoma Generate Shared Immunogenic Tumor-Specific Neoepitopes. Cancer Discov. 2021 Aug;11(8):1938-1951. doi:10.1158/2159-8290.CD-20-0555
The project aim at identifying tumor-specific neo-Ag derived from RNA processing or expression in human cancers. The in silico predicted neo-Ag will be further evaluated using immune-peptidomic. After definition of the most promising epitopes, the spontaneous response they induce in patients and their putative immunogenicity will be determined. The induction of an immune response in PBMCs from healthy donor or cancer patients and/or recognition by specific CD8 T cell clones will be assessed. The candidate will work in close collaboration on this research program with a postdoctoral fellow and a research engineer.
- Design and perform in vitro experiments: cell separation, cell culture, co-culture, multiparametric flow cytometry, cell sorting, Elispot, protein measurements
- Perform data analysis
- Technological and bibliographic watch
- Presentation of your results, reporting.
Training and experience required
- Master2 in Immunology, Molecular and Cellular Biology, or Biotechnology
- Knowledge in human immunology, oncology and immunotherapies and some expertise in flow cytometry will be appreciated
- Scientific rigor and excellent analytical and synthetic skills
- Team player and dynamic personality with passion for innovation and problem solving
- Good proficiency of English.
All our opportunities are open to people with disabilities
- Type of contract: COD
- Starting date: As soon as possible
- Duration: 18 months (to 3 years)
- Working time: Full time
- Remuneration: According to the current grids
- Benefits: Collective catering, reimbursement of transportation fees up to 70%, supplementary health insurance
- Location of the position: Paris
- Reference: 2022-08-U932-ING02
Please send your CV, letter of motivation and 2 references, to email@example.com
Publication date: August 2022
Deadline for application: September 2022
Institut Curie is an inclusive, equal opportunity employer
and is dedicated to the highest standards of research integrity.