Offre de thèse H/F « Rôle des voies intracellulaires exploitées par les virus dans la sécrétion de vésicules extracellulaires par les cellules tumorales et leurs fonctions immunitaires »

Institut Curie Siège
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Date de clôture
Lieu(x) de travail
Paris
Secteur
Centre de Recherche
CDD
The Institut Curie is a major player in the research and fight against cancer. It consists of a hospital and a Research Center of more than 1000 employees with a strong international representativeness.
The objective of the Curie Institute Research Center is to develop basic research and to use the knowledge produced to improve the diagnosis, prognosis, and therapeutics of cancers as part of the continuum between basic research and innovation serving the patient

 

Laboratory

 

Description of the PhD thesis project

Cells release membrane-enclosed extracellular vesicles (EVs), which act as intercellular messengers, by transferring surface or internal signals into surrounding cells. The host laboratory aims at unraveling the diversity of EVs, with a goal to identify EVs able to induce anti-tumor immune responses. It has identified two proteins whose expression in virus-infected cells may change the downstream immune functions of their EVs. This project will explore this hypothesis in human cancer. Expression of the identified targets will be manipulated in breast cancer cell lines, and the consequences on EV release, tumor growth and immune responses will be evaluated. A long-term goal is to identify EVs with potential as anti-tumor immunotherapies in breast cancer.

 

Recent publications:

 

  1. M Mathieu, N Névo, M Jouve, J I Valenzuela, M Maurin, F Verweij, R Palmulli, D Lankar, F Dingli, D Loew, E Rubinstein, G Boncompain, F Perez, C Théry (2021). Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. Nat Commun 12: 4389.

 

  1. L Martin-Jaular, N Nevo*, J Schessner*, M Tkach, M Jouve, F Dingli, D Loew, K W. Witwer, M Ostrowski, G H. H. Borner *, C Théry * (2021). Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO J e105492.

 

  1. F Cocozza*, N Névo*, E Piovesana*, X Lahaye, J Buchrieser, O Schwartz, N Manel, M Tkach*, C Théry*, L Martin-Jaular*. (2020). Extracellular vesicles containing ACE2 efficiently prevent infection by SARS-CoV-2 Spike protein-containing virus. J Extracell Vesicles 10: e12050.

 

  1. L Liao*, L Martin-Jaular*, E Soueidi, M Jouve, D C. Muth, T H. Schoyen, T Seale, N J. Haughey, M Ostrowski, C Théry*, K W. Witwer* (2019). Acetylcholinesterase is not a generic marker of extracellular vesicles. J Extracell Vesicles, 8: 1628592.

 

  1. M Tkach, J Kowal, A E. Zucchetti, L Enserink, M Jouve, D Lankar, M Saitakis, L Martin-Jaular, C Théry (2017). Qualitative differences in T cell activation by dendritic cell-derived extracellular vesicle subtypes. EMBO J, 36: 3012-3028.

 

 

International, interdisciplinary & intersectoral aspects of the project

  • International: This collaborator will mentor the PhD student, and thus provide strong international and intersectoral exposure.
  • Intersectoral: The project involves an international collaboration with a long-term collaborator, who is also involved in development of a biotech company in his country.
  • Interdisciplinary: This biology project will require the student to acquire expertise in bioinformatics, to analyse independently the -omics dataset generated, and to interact with physicians of the Institut Curie, to obtain and analyse clinical samples, thus to develop his/her interdisciplinary skills.

 

 

Candidate Profile

 

  • Applicants should have a strong desire to explore cell biological phenomena, and should show solid capacity for independent and creative thinking.
  • Background in cell biology, and/or immunology is strongly recommended.
  • Background in extracellular vesicles and bioinformatics is a plus but not compulsory.
  • The project highly relies on biochemistry and flow cytometry techniques, for which the applicant should have either experience or a strong motivation to learn.

 

All our opportunities are open to people with disabilities

 

 

Contract information

 

  • Type of contract: Fixed-term contract
  • Starting date:  September 1st, 2022
  • Duration: 3 years
  • Working time: full time
  • Remuneration: 2.123€ gross / month
  • Benefits: Collective catering, reimbursement of transportation fees up to 70%, supplementary health insurance
  • Location of the position: Paris

 

Contact

 

Application process

Only via the online application (https://training.institut-curie.org/eureca). Email applications are not accepted.

 

To know more about the eligibility criteria and the online application process, please check the website https://training.institut-curie.org/eureca or contact phd.eureca@curie.fr

 

Publication date: 20/12/2021

Deadline for application: 10/01/2022.

Exemple : +33112365489.
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