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Biogenesis and functions of Extracellular vesicles in intercellular communication

image projet 2 equipe Raposo

 

Extracellular vesicles are a heterogeneous group of cell-derived membranous structures comprising exosomes and microvesicles, which originate from the endosomal system or are shed from the plasma membrane, respectively. They are present in biological fluids and are involved in multiple physiological and pathological processes. Extracellular vesicles are considered as an additional mechanism for intercellular communication, allowing cells to exchange proteins, lipids and genetic material. Knowledge of the cellular processes that govern extracellular vesicle biology is essential to shed light on the physiological and pathological functions of these vesicles as well as on clinical applications involving their use and/or analysis. In particular, we study:

 

  • The cellular and molecular mechanisms of biogenesis of exosomes and microvesicles

-  How the different Extracellular Vesicles contribute to the communication between Melanocytes and Keratinocytes in the skin

-   The cellular and molecular mechanisms involved in the EV-based intercellular communication loop and the impact on cell physiology and function

 

Main contributions (last 5 years):

           

 

Hurbain,I., Macé,A.S., Romao, M., Sengmanivong,L., Ruel, L., Basto,R., Thérond,P.P., Raposo, G. and D’Angelo, G.2021. Microvilli-derived Extracellular Vesicles Govern Morphogenesis in Drosophila wing epithelium  bioRxiv.11.01.363697; doi: https://doi.org/10.1101/2020.11.01.363697

Raposo G, van Niel G, Stahl PD (2021). Extracellular vesicles and homeostasis-An emerging field in bioscience research.  FASEB Bioadv. 2021 Mar 17;3(6):456-458. doi: 10.1096/fba.2021-00009

Verweij, F.J., Revenu, C., Arras, G., Dingli, F., Loew, D., Pegtel, D.M., Follain, G., Allio, G., Goetz, J.G., Zimmermann, P., et al. 2019. Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo. Dev Cell 48, 573-589 e574.

Stahl, P.D., and Raposo, G. 2019. Extracellular Vesicles: Exosomes and Microvesicles, Integrators of Homeostasis. Physiology (Bethesda) 34, 169-177. doi: 10.1152/physiol.00045.2018.

Raposo, G., and Stahl, P.D. (2019). Extracellular vesicles: a new communication paradigm? Nat Rev Mol Cell Biol. (9):509-510. doi: 10.1038/s41580-019-0158-7.

Verweij, F.J., Bebelman, M.P., Jimenez, C.R., Garcia-Vallejo, J.J.,Janssen, H., Neefjes, J., Knol, J.C., de Goeij-de Haas, R., Piersma, S.R., Baglio, S.R., et al. (2018). Quantifying exosome secretion from single cells reveals a modulatory role for GPCR signaling. J Cell Biol 217, 1129-1142.

van Niel, G., D'Angelo, G., and Raposo, G. (2018). Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol 19, 213-228.

Stahl, P.D., and Raposo, G. 2018. Exosomes and extracellular vesicles: the path forward. Essays Biochem 62, 119-124.

Lo Cicero, A., Delevoye, C., Gilles-Marsens, F., Loew, D., Dingli, F., Guere, C., Andre, N., Vie, K., van Niel, G., and Raposo, G. 2015. Exosomes released by keratinocytes modulate melanocyte pigmentation. Nat Commun 6, 7506.