Exploiting State of the Art Imaging

Our scientific projects require a tight complementarity between different cell biological methods, with the cellular imaging at different scales as central piece of our research. Over the years, we have developed and exploited different electron microscopical methods by Transmission Electron Microscopy (TEM), like conventional EM after chemical fixation or quick physical immobilization by High Pressure Freezing (HPF), the immunogold labeling on ultrathin cryosections, or the electron tomography. We are actively involved in combining optical microscopy with electron microscopy, the so called Correlative Light Electron Microscopy (CLEM). Such expertise conducted to the establishment of multiple collaborations in France and abroad. 

Main Publications:

Adams S.D, Csere J, D’Angelo G,  Romao, M.,Carter EP, Arnandis T, Dodel M, Kocher H, Grose R, Raposo G, Mardakheh F, Godinho SA (2021). Centrosome amplification mediates small extracellular vesicles secretion via lysosome disruption. Curr Biol. 2021 Apr 12;31(7):1403-1416.e7. doi: 10.1016/j.cub.2021.01.028

Figon, F., Hurbain,I., Heiligenstein,X., Trépout,S.,Medjoubi,K., Somogyi, A., Delevoye,C., Raposo, G. and Casas,J. 2021. bioRxiv 2021. Catabolism of lysosome-related organelles in color-changing spiders supports intracellular turnover of pigments 02.22.432296; doi: https://doi.org/10.1101/2021.02.22.432296

Bergamelli M, Martin H, Bénard M, Ausseil J, Mansuy JM, Hurbain I, Mouysset M, Groussolles M, Cartron G, Tanguy le Gac Y, Moinard N, Suberbielle E, Izopet J, Tscherning C, Raposo G, Gonzalez-Dunia D, D’Angelo C, Malnou CE 2020. Human cytomegalovirus infection changes the pattern of surface markers of small extracellular vesicles isolated from first trimester placental histocultures. bioRxiv 2020.11.30.402693; doi: 10.1101/2020.11.30.402693

Peterman, E., Gibieza, P., Schafer, J., Skeberdis, V.A., Kaupinis, A., Valius, M., Heiligenstein, X., Hurbain, I., Raposo, G., and Prekeris, R. (2019). The post-abscission midbody is an intracellular signaling organelle that regulates cell proliferation. Nat Commun 10, 3181.

Hurbain, I., Romao, M., Bergam, P., Heiligenstein, X., and Raposo, G. (2017). Analyzing Lysosome-Related Organelles by Electron Microscopy. Methods Mol Biol 1594, 43-71.

Heiligenstein, X., Paul-Gilloteaux, P., Raposo, G., and Salamero, J. (2017). eC-CLEM: A multidimension, multimodel software to correlate intermodal images with a focus on light and electron microscopy. Methods Cell Biol 140, 335-352.

Heiligenstein, X., Hurbain, I., Delevoye, C., Salamero, J., Antony, C., and Raposo, G. (2014).

Step by step manipulation of the CryoCapsule with HPM high pressure freezers. Methods Cell Biol 124, 259-274.