Cell-free reconstitution reveals centriole cartwheel assembly mechanisms
Authors
P. Guichard, V. Hamel, M. Le Guennec, N. Banterle, I. Iacovache, V. Nemčíková, I. Flückiger, K. N. Goldie, H. Stahlberg, D. Lévy, B. Zuber, P. Gönczy
Abstract
Abstract
How cellular organelles assemble is a fundamental question in biology. The centriole organelle organizes around a nine-fold symmetrical cartwheel structure typically ∼100 nm high comprising a stack of rings that each accommodates nine homodimers of SAS-6 proteins. Whether nine-fold symmetrical ring-like assemblies of SAS-6 proteins harbour more peripheral cartwheel elements is unclear. Furthermore, the mechanisms governing ring stacking are not known. Here we develop a cell-free reconstitution system for core cartwheel assembly. Using cryo-electron tomography, we uncover that the