Combining Spinach-tagged RNA and gene localization to image gene expression in live yeast
Authors
David Guet, Laura T. Burns, Suman Maji, Jérôme Boulanger, Pascal Hersen, Susan R. Wente, Jean Salamero, Catherine Dargemont
Abstract
Abstract
Although many factors required for the formation of export-competent mRNPs have been described, an integrative view of the spatiotemporal coordinated cascade leading mRNPs from their site of transcription to their site of nuclear exit, at a single cell level, is still partially missing due to technological limitations. Here we report that the RNA Spinach aptamer is a powerful tool for mRNA imaging in live