• Home >
  • Publications >
  • Constitutive Siglec-1 expression by human dendritic cell precursors enables HIV-1 replication and transmission

Constitutive Siglec-1 expression by human dendritic cell precursors enables HIV-1 replication and transmission

24 Mar 2019bioRxiv

DOI : 10.1101/578377

Authors

Nicolas Ruffin, Ester Gea-Mallorquí, Flavien Brouiller, Mabel Jouve, Aymeric Silvin, Peter See, Charles-Antoine Dutertre, Florent Ginhoux, Philippe Benaroch

Abstract

Abstract

Human dendritic cell (DC) lineage has been recently unraveled by high dimensional mapping revealing the existence of a discrete new population of blood circulating DC precursor (pre-DC also referred to as AS DC). Among all blood DC subsets, only pre-DC highly express Siglec-1, a lectin-like receptor able to bind HIV-1. We show that pre-DC are uniquely equipped among blood DC populations to promote HIV-1 replication and dissemination. Pre-DC stands out as the most susceptible DC population to infection by both HIV-1 CXCR4- and CCR5-tropic viral particles in a Siglec-1-dependent manner. HIV-1-infected pre-DC produce new viral progeny and transmit the virus to CD4+T cells. Upon TLR activation, pre-DC become resistant to HIV-1 fusion and thus to infection and switch to a replication-independent mechanism of virus transfer to activated primary T lymphocytes mediated by Siglec-1. Thus, beside their role in DC ontogeny, blood pre-DC possess stage-specific properties that HIV-1 may exploit for viral spreading and modulation of the immune response.

Members

PHILIPPE BENAROCH

Directeur de recherche CNRS