Mapping intracellular mechanics on micropatterned substrates

Significance

Cell mechanics is crucial for many, if not all, cell functions. Although mechanics at the scale of the whole cell is extensively documented, there are only few methods to measure intracellular mechanics at the local scale. Here, we develop a technique that allows us ( i ) to map the spatial variations of intracellular mechanical parameters; ( ii ) to study how the actin and microtubule cytoskeleton, intracellular membranes, and ATP-dependent active forces contribute to intracellular mechanics; and ( iii ) to differentiate normal and cancer cells. Because intracellular mechanical maps can detect subtle differences in the spatial distribution of mechanical parameters even in the absence of any change in their average values, our approach could provide a diagnostic and prognostic tool for cancers.