S-phase chromatin dynamics in Physarum polycephalum

During the S-phase of the cell cycle, genetic material is replicated and packaged into chromatin. This requires the deposition of newly synthesized histones for the maintenance of the architecture. Using the unusual feature of incorporation of exogenous proteins within the naturally synchronous syncytial cell of Physarum polycephalum, tagged exogenous proteins can be tracked at specific stages of the cell cycle. Besides the introduction of the model system, we will examine the usage of H3 variants throughout the S-phase of Physarum and the effects of the incorporation of trace amounts of H3K27M on the epigenetic landscape.