Ingénieur d’étude en recombinaison homologue (F/H)

The laboratory

The successful candidate will join the Compartmentalization and Dynamics of Nuclear Functions team led by Angela Taddei at Institut Curie (https://curie.fr/equipe/taddei). Our research focuses on understanding how nuclear organization regulates genome functions, with a particular emphasis on homologous recombination. We combine state-of-the-art in vivo quantitative microscopy approaches, including super-resolution imaging, with genetic and molecular biology techniques.

The project

This position is part of a project aimed at studying the various stages of the DNA damage response (DDR) in relation to genome spatial organization in the yeast S. cerevisiae.

The missions :

The recruited engineer will have two main responsabilities :

-         The individual will strengthen the team’s capacity to generate new yeast strains required for the project’s progress (tool construction : plasmids and strains)

-         He/she will adapt the oligopaint tcehnique to vizualize 3D chromosome conformation during the DNA damage response. This part of the project will be carried out with the assistance of a reserach engineer from the Taddei team and a research engineer from the Curie Coretech platform.

As part of his duties, the engineer will conduct experiments, analyzeresults, maintain an up-to-date electronic laboratory notebook, and present project progress at laboratory meetings.