CurieCoreTech - Cytometry (CYTPIC)

The cytometry platform is organizing a training/hands on for FlowJo software in the BDD amphitheater (Institut Curie Paris) on September 8, 2023. The day presented by Serena Di Cecilia (Treestar/Becton Dickinson) will be divided into two sessions as detailed below. You have the possibility to come with your laptop and flowjo installed on it (demo version) to practice in real time.

Registration is free but mandatory via the following link: https://forms.office.com/e/ku0EL5nSy0

FlowJo Training agenda:

Basic: 9:30-12:30

Navigating the v10 Workspace; Customizing Ribbons; Preferences (customize fonts, gating, cytometer setting, computer performance); Creating and Editing Groups; Keywords; Sample quality check and exclusion gates; Graphs, Gating and Ancestry; The Layout Editor; Batching and Exporting Graphics; The Table Editor and Exporting Statistics; Compensation; Demonstration.

Advanced: 2:00-4:00

High Dimensional data analysis; The FlowJo Plugins; Installation; Quality Check plugins: (FlowAI, FlowClean); Reduce Dimensionality, tSNE, UMAP, TriMap; Automated clutering and more : FlowSOM, x-shift, Phenograph; Exploring Deeper: Hyperfinder, cluster explorer, ViolinBox; Demonstration.

10XGenomics Chromium Controller with Next GEM Accessory Kit of the CYTPIC (CYTometry Platform Institut Curie) for sale :

The 10XGenomics Chromium Controller, based on Next GEM technology, is a microfluidic instrument for isolating cells or cell nuclei from a suspension (biological sample) for transcriptomic, proteomic and/or epigenetic analysis in NGS at the scale of single cells or nuclei. This instrument can capture molecular readouts of cell activity in multiple dimensions and have the ability to generate tens of thousands of single cell partitions, each containing an identifying barcode for downstream analysis.

Instrument : Chromium Controller - product code: 1000204

Detailed description: https://www.10xgenomics.com/instruments/chromium-controller

Maintenance : Under warranty the first year and maintenance contract since end of the warranty (current contract ends on 31/12/2023)

Accessory Kit : Chromium Next GEM Accessory Kit provided - product code: 1000142

Purchase date : 2021, June

Price : 15 000€

Availability : ASAP

Please contact coralie.guerin@curie.fr for more information.

CYTPIC is pleased to announce the arrival of Anne-Gaelle Lafont, PhD.

"Since obtaining my PhD degree in biology in 2006, I have had the opportunity to work in various fields of research. I worked mainly on aquatic models (fish, cephalopods) and on the impact of environmental factors (salinity, temperature), hormones and peptides, on the physiological regulations of these different models. I also investigated the evolution history of gene families in vertebrates. For all this, I used molecular biology, cell biology, biochemistry and in silico approaches.

In 2019, I graduated from the inter university degree “Project Manager and Investigator of Clinical Trials” at Pierre and Marie Curie University. I was then recruited as a research engineer, operational manager of the biobank of the Saint-Antoine hospital in Paris. During this contract, I notably set up the microbiology laboratory for the new activity of the Biobank dedicated to research on the Intestinal Microbiota.

Since the 17th of July, I have joined the Paris Cytometry Plateform of the Curie Institute.

Anne-Gaelle Lafont."

The Cytometry Platform of the Institut Curie (CYTPIC) is happy to announce the upgrade of our Chromium Controller to the Chromium iX at the site of Orsay thanks to the Initiative Single Cell (NGS platform) and the support of U1021, UMR3348 and the Research Center.

The Chromium iX, based on NextGEM technology, is a microfluidic instrument for isolating cells or cell nuclei from a suspension (biological sample) for transcriptomic, proteomic and/or epigenetic analysis in NGS at the scale of single cells or nuclei.

The Chromium iX supports new 10X solutions (RNA Fixed, and more to come). Possible and non-exhaustive applications:

- Single Cell Gene Expression on all types of fresh or fixed cells.

- Characterization of accessible regions of chromatin (ATAC) at single nucleus scale, possible in combination with gene expression on single cells or nuclei.

- Amplification of VDJ sequences in fresh human and murine T and B lymphocytes for single-cell immune profiling of test organisms.

Cytometry platform is pleased to organize two consecutive seminars on multiplexing technologies for protein assays compatible with our Bioplex 200 instrument already available on the Paris site.

SEMINAR 2 :

Second seminar will be co-organized with ThermoFisher on June 21st from 10am to 11am in Amphithéatre Marie Curie.

Procartaplex: Detect and quantify simultaneoulsy multiple proteins (up to 80) in a single sample, using the Luminex xMAP technology. Combine multipexing efficacy with Elisa easiness, sensitivity,

precision and reproducibility. www.thermofisher.com/procartaplex

Branched DNA (bDNA): A unique approach allowing RNA detection by using successive nucleic acids hybridization

and signal amplification.

QuantiGene®plex assays: Detect and quantify multiple RNA in one sample. No need to extract or purify , no need to check RNA quality (up to 80 targets). Work on degraded samples.

Combine Branched DNA principle with Luminex® xMAP® technology.

Cytometry platform is pleased to organize two consecutive seminars on multiplexing technologies for protein assays compatible with our Bioplex instrument already available on the Paris site.

SEMINAR 1 : 

The first seminar will be co-organized with Merck on June 19th from 1pm to 2pm in Amphithéatre Marie Curie.

Cytometry Platform of Institut Curie & Luminex :  An immunoassay Platform Solution that can empower Immuno-Oncology breakthroughs

Don’t miss this unique opportunity to learn on how the Immunoassay Platform Solutions (IPS) available within the Cytometry Platform of Institut Curie can empower research into Immuno-oncology.

During this session, Merck will deep dive into their comprehensive IPS portfolio, presenting the power of the MILLIPLEX ® multiplex cancer assays compatible with Luminex technology already available within the Cytometry Platform. This multiplex solution allows up to 100 ELISA at once with high consistency, high-quality results while saving time, labor and cost.

Join us and see how these technology can help drive breakthroughs in cancer research.