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Dynamic palmitoylation events following T-cell receptor signaling

1 Dec 2020Communications Biology

DOI : 10.1038/s42003-020-1063-5

Authors

Eliot Morrison, Tatjana Wegner, Andres Ernesto Zucchetti, Miguel Álvaro-Benito, Ashley Zheng, Stefanie Kliche, Eberhard Krause, Britta Brügger, Claire Hivroz, Christian Freund

Abstract

Abstract

Palmitoylation is the reversible addition of palmitate to cysteine via a thioester linkage. The reversible nature of this modification makes it a prime candidate as a mechanism for regulating signal transduction in T-cell receptor signaling. Following stimulation of the T-cell receptor we find a number of proteins are newly palmitoylated, including those involved in vesicle-mediated transport and Ras signal transduction. Among these stimulation-dependent palmitoylation targets are the v-SNARE VAMP7, important for docking of vesicular LAT during TCR signaling, and the largely undescribed palmitoyl acyltransferase DHHC18 that is expressed in two isoforms in T cells. Using our newly developed On-Plate Palmitoylation Assay (OPPA), we show DHHC18 is capable of palmitoylating VAMP7 at Cys183. Cellular imaging shows that the palmitoylation-deficient protein fails to be retained at the Golgi and to localize to the immune synapse upon T cell activation.

Members

CLAIRE HIVROZ

Directeur de recherche Inserm