Study Engineer (F/M)

Description of position

Laboratory

Institut Curie, CNRS UMR 144 – team Daniele Fachinetti: Molecular Mechanisms of Chromosome Dynamics

Missions

Our team Molecular Mechanisms of Chromosome Dynamics, led by Daniele Fachinetti, investigates the fundamental principles that ensure the faithful transmission of genetic material in mammalian cells. We combine cell biology, genetics, epigenetics, biochemistry, and imaging to understand how centromeres are established, maintained, and function throughout the cell cycle. We also study how their deregulation can lead to genome instability – a key driver of many pathologies, including cancer.

Our team is seeking a motivated Study Engineer (IE) in Biology to contribute to a project investigating the role of aneuploidy, centromeres, and germline deletions in the transition from normal to tumoral states in neuroblastoma. The project focuses on human induced pluripotent stem cells (hiPSCs), hiPSC-derived sympathoblasts, and neuroblastoma cell lines to better understand mechanisms of tumor initiation and progression.

The successful candidate will be responsible for:

-       Maintenance and differentiation of hiPSCs and culture of several cell lines.

-       Performing transfection experiments and molecular assays (including CRISPR technology) to modulate and analyze gene expression.

-       Conducting DNA, RNA, and protein extraction followed by downstream analyses (PCR, RT-qPCR, western blot).

-       Applying molecular biology techniques such as cloning and plasmid manipulation.

-       Collaborating closely with researchers to analyze experimental data and contribute to the advancement of the project.