Engineer – Production and Characterization of Recombinant Proteins (F/M)

Laboratory:

The CNRS UMR144 "CELL BIOLOGY AND CANCER", investigates cell compartment biogenesis and molecular mechanisms governing both normal cellular functions and pathologies. Within this context, the "INTRACELLULAR TRAFFICKING: ENGINEERING AND MECHANISMS" team studies intra-cellular trafficking in mammalian cells and contributes to the development of biotechnological tools in cell biology (https://institut-curie.org/team/perez).

Position Overview:

We are seeking an Engineer with a background in biochemistry and molecular biology to contribute to ongoing scientific projects. These projects are focused on the biochemical and structural characterization of medically important proteins, including human protein fragments, single domain antibodies, artificially designed proteins, growth factors, and more. The role will involve the production of recombinant proteins and protein complexes using established protocols, as well as the development of new methodologies. Additionally, responsibilities include plasmid, cell and protein inventory management, media and reagent preparation, lab documentation, troubleshooting, and adaptation to the collaborative laboratory environment. There are opportunities for cross-training in complementary techniques if required for specific projects.

Key Responsibilities:

-        Amplification, purification, and characterization of plasmid DNA.

-        Molecular (sub)-cloning, ideally with independent construct design.

-        Expression and purification of recombinant proteins at analytical and preparative scales.

-        Preparation of samples for in vitro protein binding assays.

-        Detailed record-keeping and report generation for all procedures and results.

-        Summarization and presentation of data at internal meetings.

-        Contribute to preparation and maintaining of laboratory stocks.