Single cell chromatin immunoprecipitation (scChIP) allows genome-wide mapping of histone modifications in single cells. One can study how these marks define subpopulations of cells and impact on phenotype and gene transcription.

For scChIP-seq, single cells are first isolated into droplets containing lysis buffer and MNase (to digest chromatin), using the microfluidics platform available at the Custom Single Cell Omics platform. Droplets are then fused to a droplet carrying distinct oligonucleotides for barcoding of individual cells. Then, droplets are pooled and chromatin immunoprecipitation is performed. Library preparation is carried out and sequencing is performed at the NGS platform. For more information, see Grosselin et al 2019 (PMID: 31152164).

For the moment, the Custom Single Cell Omics platform focusses on two histone marks: trimethylation of H3K27 (repressive mark) and trimethylation of H3K4 (permissive mark). Other marks might be available in the near future. Based on high-throughput microfluidics, the scChiP is adapted to samples of more than 300,000 freshly dissociated cells, in order to obtain a profile for more than 1000 cells / epigenetic mark.

Workflow scChIP-seq