Postdoctoral Position in Molecular & Cellular Genetics (M/F)

Laboratory

Our research team is interested in the spatio-temporal regulation of the mechanisms that govern genome stability in response to perturbations to the DNA replication process. Flaws in the DNA replication process, known as replication stress (RS), is a source of genome instability contributing to genomic disorders including cancer1. The causes of replication stress are many and varied but ultimately result in stressed replication forks that are fragile DNA structures prone to chromosomal breakage, rearrangement and mutation2. Our team aims to understand the spatial and temporal organization of molecular circuits that prevent stressed forks from being converted into pathological DNA structures. We investigate how recombination, repair, chromatin and RNA-based processes prevent replication stress-induced genome instability within the sub-nuclear architecture of the genome, using the yeast S. pombe and human cells as model systems. We combine genetic, cellular, genomic and molecular approaches to tackle these questions. Our team is based at the Institut Curie, on the Orsay site, about 20 km south of Paris. Our team is looking for a highly motivated post-doctoral researcher keen to understand how nuclear organization contributes to maintaining genome stability in response to replication perturbations. The candidate will join a research team of around 8 people, made up of PhD students, post-docs, engineers and researchers, to carry out his or her research in a collaborative and interactive spirit, while encouraging the taking of responsibility and initiative.

Team web site: https://institut-curie.org/team/lambert

The project

The research project aims to decipher how nuclear architecture contributes to the maintenance of genome stability in response to replication perturbations. Our team has identified the nuclear pore complex as a molecular hub for promoting replication fork repair in the yeast S. pombe. The research project aims to understand the spatial specification of replication fork integrity pathways. The aim is to determine whether all nuclear compartments have the same capacity to preserve genome stability in response to replication perturbations, and to identify the underlying molecular mechanisms. The project requires the implementation of innovative proteomic and genomic approaches, and is part of a consortium (Space-ForkIn) funded by the ANR.

Bibliographical references

1.       Kramarz et al. Nature Communications 2020 DOI: 10.1016/j.celrep.2020.02.065

2.       Schirmeisen et al. Genes DOI: 10.3390/genes12122010

3.       Schirmeisen et al. Biorxiv 2023DOI:10.1101/2023.11.13.

4.       Audoynaud et al. Mol Cell 2023 DOI: 10.1016/j.molcel.2023.02.

5.       Schirmeisen et al. DNA repair 2023 DOI: 10.1016/j.dnarep.2023.103

6.       Ouasti et al. Elife 2024 DOI:10.7554/elife.91461.1  

Contraints and risks

-          Use of biological/chemical products

Training and Skills required

-          Candidate should hold a PhD in biology, cellular biology, or related areas.

-          Fresh PhD graduate or with up to 3 years of postdoctoral experience.

-          Applicants are invited to indicate the specific level of experience is these subjects.

o    Expertise in yeast genetics

o    Scientific expertise in the field of genome stability

o    Expertise in molecular cell biology and proteomics

o    Expertise in analyzing genomics data

-          Language skills: Fluency in English level and communication skills. French is a plus.

Abiliites

-          Ability to work independently, managerial abilities, aptitude for working in a team.

-          The candidate should be highly motivated, curious, and enthusiastic to work in a collaborative team.

All our opportunities are open to people with disabilities

Contract information

Type of contract: Fixed-term contract.

Starting date:  As soon as possible

Duration: 1 year (renewable 1 year)

Working time: full time

Remuneration: according to the current grids

Benefits: Collective catering, reimbursement of transportation fees up to 70%, supplementary health insurance

Location of the position: Orsay

Reference: NA

Contact

To apply, please send your CV, list of publications and cover letter.

The cover letter should clearly state the candidate's motivation and scientific curiosity for the project. The application should include at least two names of scientific personalities likely to recommend the candidate. CVs will be processed on a rolling basis, so the position may be filled before the application deadline.

Publication date: 10/06/2024

Deadline for application: 01/10/2024

Institut Curie is an inclusive, equal opportunity employer

and is dedicated to the highest standards of research integrity.

https://euraxess.ec.europa.eu/sites/default/files/brochures/eur_21620_en-fr.pdf