Team

Cell Biology of Mammalian Neurogenesis

ALEXANDRE BAFFET

Team Leader

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Unit

Cell Biology and Cancer (UMR144)

Thematic areas of research:

Cell and developmental biology

Other teams

GIF composé de differentes images montrant des organoides et du tissus fetal immuno-marqué

Team Baffet

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Team

ALEXANDRE BAFFET

Cell Biology of Mammalian Neurogenesis

The neocortex is the center for higher brain functions, such as perception, decision-making and language. Our group focuses on the mechanisms governing neocortex development, with a strong interest on the role and regulation of the neural stem cells.

Members

Post-Doctoral Researcher

AMANDINE DI CICCO

Engineer

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Past members

Jean-Baptiste Brault

Post-Doctoral Researcher

Sarah Farcy

PhD Student

Jacopo Carpentieri

PhD Student

Laure Coquand

PhD Student

Microscopy Images

Centrosomes dans les glies radiaires d'un organoide

Neocortical Organoids

Human Fetal Brain

Previous works

Schematic representation of correlative microscopy pipeline. B. Step-by-step protocol for semi-automated correlative microscopy. (1) bRG cells are live imaged for 48 hours. (2) 4X brightfield images containing the video coordinates are assembled. (3) Organoid slices are fixed, immunostained for SOX2, EOMES and NEUN and imaged. (4) Images are automatically segmented to outline slices from live and fixed samples. (5) Slice contours are automatically paired based on shape and area and (6) aligned (including a

A cell fate decision map reveals abundant direct neurogenesis in the human developing neocortex

A. WDR81-/- postnatal day 7 brains are microcephalic and display reduced cortical surface area as compared to WT brains. B. Quantification of hemisphere area at P7 in WT and WDR81 KO1 brains. C. DAPI staining of P7 WT and WDR81-/- cross sections reveals reduced cortical thickness in mutants. D. Quantification of cortical thickness in WT, KO1 and KO2 brains at P0 and P7. E. NeuN staining of WT and WDR81-/- cortical plates (CP) at P0. F. Quantification of NEUN+ cells in WT and WDR81 KO1 cortical plates at P0

Endosomal trafficking defects cause microcephaly

RUSH assay for CRB3-GFP in control (mcherry) and dynactin-inhibited radial glial cells (CC1-p150-dsRed), electroporated at E.15.5 and imaged at E16.5. CRB3 is retained in the endoplasmic reticulum until the addition of biotin, which releases it for trafficking. Upon dynein/dynactin inhibition, Crumbs fails to reach the apical surface. SBP: Streptavidin-binding protein. St: Streptavidin. Scale bar =

RAB6 and dynein transport prevents neuronal progenitor delamination

(Left) Live imaging of CAMSAP3-GFP and EB3-mCherry in mouse aRG cell basal process, and corresponding kymograph. (Right) The microtubule network of the apical process of aRG cells is unipolar, with the minus ends concentrated apically, and the plus ends growing in the basal direction. The mother centriole serves as a template for the primary cilium, which extends into the ventricle. A second ring-like microtubule network is present at the apical endfoot (PMID: 32238932). In the basal process of both aRG and

CAMSAPs organize an acentrosomal microtubule network from basal varicosities in radial glial cells

cellules souches neurales infectees par le virus ZIKA

Neural Stem Cell Tropism of Zika Virus

Key publications

2023

The microtubule cytoskeleton of radial glial progenitor cells
Current Opinion in Neurobiology

Ryszard Wimmer, Alexandre D. Baffet
(2023 01 Jun)
2022

RAB6 and dynein drive post-Golgi apical transport to prevent neuronal progenitor delamination
EMBO reports

Jean‐Baptiste Brault, Sabine Bardin, Marusa Lampic, Jacopo A Carpentieri, Laure Coquand, Maxime Penisson, Hugo Lachuer, Guiliana Soraya Victoria, Sarah Baloul, Fatima El Marjou, Gaelle Boncompain, Stephanie Miserey‐Lenkei, Richard Belvindrah, Vincent Fraisier, Fiona Francis, Franck Perez, Bruno Goud, Alexandre D Baffet
(2022 06 Oct)
2022

Endosomal trafficking defects alter neural progenitor proliferation and cause microcephaly
Nature Communications

Jacopo A. Carpentieri, Amandine Di Cicco, Marusa Lampic, David Andreau, Laurence Del Maestro, Fatima El Marjou, Laure Coquand, Nadia Bahi-Buisson, Jean-Baptiste Brault, Alexandre D. Baffet
(2022 10 Jan)
2021

CAMSAPs organize an acentrosomal microtubule network from basal varicosities in radial glial cells
Journal of Cell Biology

Laure Coquand, Guiliana Soraya Victoria, Alice Tata, Jacopo Amerigo Carpentieri, Jean-Baptiste Brault, Fabien Guimiot, Vincent Fraisier, Alexandre D. Baffet
(2021 02 Aug)

All publications

News

Congratulations to Dr. Alexandre Baffet, 2023 winner of the Fondation Bettencourt Schueller Impulscience program

Dr. Alexandre Baffet, head of research team at the Institut Curie, is one of 7 new beneficiaries of the Impulscience research support program. This 2.3 million euro grant will enable him to learn more about brain stem cells, especially in the human neocortex.

22/11/2023

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