DNA replication must adapt to changes in chromatin organization associated with cell differentiation and development, whose deregulation can challenge genome stability and leads to mutations, cancer and many other genetic diseases. However, despite intensive studies, the mechanisms that coordinate where and when replication initiates in the human genome remain poorly known. Our team focuses on using cutting-edge high-throughput genomic approaches and genome-wide data analyses to study the spatio-temporal replication program of the human genome and its impact on genome stability, in particular to address the following questions:
- What determines the replication program, i.e. the position, the time of firing and the efficiency of replication origins in the human genome?
- How this program is regulated and associated with gene transcription and chromatin organization?
- How deregulation of these programs challenges genome stability and leads to human diseases?
A next-generation method for examining DNA replication using “single molecule” analysisSuccessful replication is essential to maintaining genome integrity. At Institut Curie, a totally new “optical replication mapping” method has just been perfected by Chunlong Chen’s “Genome Replication and Instability Program” team as part of an international collaboration.13/07/2021
Genomic instability and pre-cancerous lesions: the role of R-loops gradually revealedIn the cellular construction process, DNA transcription and replication mechanisms can sometimes collide. These collisions lead to errors in DNA replication in the cell, and resulting defective cells can then lead to cancer.04/09/2020
Une origine importante de fragilité de l’ADN enfin élucidéeLes Common fragile sites (CFSs), ou sites fragiles communs en français, sont des fragments d’ADN qui peuvent facilement se casser lorsqu’elles sont soumises à de fortes contraintes, comme lors du « stress » que représente la réplication. Des chercheurs de l’Institut Curie ont étudié l’une des origines de cette instabilité.16/12/2019